利用蜱细胞培养衍生的边缘无形体抗原进行竞争ELISA法用于无形体病的血清学诊断。

Use of tick cell culture-derived Anaplasma marginale antigen in a competitive ELISA for serodiagnosis of anaplasmosis.

作者信息

Saliki J T, Blouin E F, Rodgers S J, Kocan K M

机构信息

Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater 74078, USA.

出版信息

Ann N Y Acad Sci. 1998 Jun 29;849:273-81. doi: 10.1111/j.1749-6632.1998.tb11059.x.

DOI:10.1111/j.1749-6632.1998.tb11059.x

PMID:9668475

Abstract

Anaplasma marginale was propagated in a continuous tick cell line and detergent-solubilized infected cells were used as antigen in a competitive ELISA (C-ELISA) for detection of Anaplasma-specific antibody in bovine sera. Positive control sera competed well (> or = 35% inhibition) with an A. marginale-specific monoclonal antibody for binding to this antigen, while negative sera failed to compete (< 35% inhibition). The C-ELISA was compared to the standard complement-fixation test (CFT) using 2,208 bovine sera. Overall, C-ELISA was more sensitive than CFT (24.9% versus 9.4%), mainly because CFT yielded "suspicious" or "anti-complementary" results in 10.5% of the sera and also failed to identify several vaccinated and carrier cattle that were C-ELISA-positive. The apparent agreement between CFT and C-ELISA was 89.6% and the kappa value was 0.6. These results show that this C-ELISA would be a suitable replacement of the CFT as the standard test for detection of A. marginale antibody.

摘要

边缘无形体在连续的蜱细胞系中增殖，用去污剂溶解感染细胞作为抗原，用于竞争ELISA（C-ELISA）检测牛血清中的无形体特异性抗体。阳性对照血清与边缘无形体特异性单克隆抗体竞争良好（抑制率≥35%），可结合该抗原，而阴性血清竞争失败（抑制率<35%）。使用2208份牛血清将C-ELISA与标准补体结合试验（CFT）进行比较。总体而言，C-ELISA比CFT更敏感（24.9%对9.4%），主要是因为CFT在10.5%的血清中产生了“可疑”或“抗补体”结果，并且未能识别出几只C-ELISA呈阳性的接种疫苗和携带病原体的牛。CFT与C-ELISA之间的明显一致性为89.6%，kappa值为0.6。这些结果表明，这种C-ELISA将是一种合适的替代CFT的标准试验，用于检测边缘无形体抗体。