用于检测确诊有边虫病的牛群中牛抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detecting antibodies in cattle in a herd in which anaplasmosis was diagnosed.
作者信息
Thoen C O, Blackburn B, Mills K, Lomme J, Hopkins M P
出版信息
J Clin Microbiol. 1980 May;11(5):499-502. doi: 10.1128/jcm.11.5.499-502.1980.
An enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibodies in anaplasmosis with an antigen prepared from infected bovine erythrocytes. Protein A labeled with horseradish peroxidase was used as conjugate. A comparison was made of results of ELISA, complement fixation test (CFT), and card test (CT) on sera from 97 cows in a herd in which anaplasmosis had been diagnosed. Positive ELISA reactions were observed in serum dilutions of 1:20 or greater in each of 26 cows positive by CFT and in 18 of 22 cows suspected by CFT. Of 49 cows negative by CFT, 31 were negative by ELISA. Positive ELISA reactions were observed in 45 cows positive by CT; 27 of 44 cows negative by ct were negative by ELISA. No ELISA reactions were detected in the sera of 23 cows found negative in the CFT and CT or in 8 cows negative in CFT and positive in CT. No positive CFT, CT, or ELISA reactions were observed in sera of cattle in a noninfected herd.
利用从感染牛红细胞制备的抗原开发了一种酶联免疫吸附测定（ELISA），用于检测无形体病中的抗体。用辣根过氧化物酶标记的蛋白A作为缀合物。对一个已诊断出无形体病的牛群中97头奶牛的血清进行了ELISA、补体结合试验（CFT）和卡片试验（CT）结果的比较。在CFT检测为阳性的26头奶牛中，每头奶牛血清稀释度为1:20或更高时均观察到ELISA阳性反应，在CFT怀疑为阳性的22头奶牛中有18头呈阳性。在CFT检测为阴性的49头奶牛中，31头ELISA检测也为阴性。在CT检测为阳性的45头奶牛中观察到ELISA阳性反应；在CT检测为阴性的44头奶牛中，27头ELISA检测也为阴性。在CFT和CT检测均为阴性的23头奶牛血清中以及在CFT检测为阴性而CT检测为阳性的8头奶牛血清中未检测到ELISA反应。在未感染牛群的牛血清中未观察到CFT、CT或ELISA阳性反应。
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