Gomes-Da-Silva J, Silva M C, Tavares M A
Institute of Anatomy, Medical School of Porto, Portugal.
Ann N Y Acad Sci. 1998 May 30;844:310-3.
Taking into account that methamphetamine (MA) is a popular recreational drug among young adult women, i.e., in gestational age, the present model aims to assess the effects of its exposure during development. In this experimental model, MA effects are assessed in the rat during the first month of life, regarding both general growth parameters, and gross morphological effects in the retina as part of the evaluation of sensory systems. Experimental animals were obtained from 60-day-old nulliparous females. Litters were culled to 8 pups (4 males and 4 females, whenever possible), individually marked and weighed every two days. Experimental groups received 10 mg (+)methamphetamine hydrochloride kg body weight/day, subcutaneously, twice daily, from postnatal day (PND) 1 to the day before sacrifice; control groups received isovolumetric doses of saline, in the same protocol. Pups were weaned on PND 21. Groups were sacrificed on PND 5, 7 and 30. Animals exposed to MA presented increased percentage of retinal hemorrhages (18, 7 and 11% on PND 5, 7 and 30, respectively) compound to control groups (2% on PND 7, 0% on PND 5 and 30). On PND 30, the mean body weight of males exposed to MA was 75% of the mean weight of male controls, whereas for females, mean body weights were 70% of those of female controls. These findings support the view that developmental parameters in the rat are deleteriously affected by early exposure to MA.
考虑到甲基苯丙胺（MA）是年轻成年女性（即处于妊娠期）中一种流行的消遣性药物，本模型旨在评估其在发育过程中暴露的影响。在这个实验模型中，在大鼠出生后的第一个月内评估MA的影响，涉及一般生长参数以及作为感觉系统评估一部分的视网膜大体形态学影响。实验动物取自60日龄未生育的雌性大鼠。将每窝幼崽挑选至8只幼崽（尽可能为4只雄性和4只雌性），每两天对其进行个体标记并称重。实验组从出生后第1天（PND）至处死前一天，每天皮下注射10mg盐酸（+）甲基苯丙胺/千克体重，每日两次；对照组按照相同方案接受等体积的生理盐水。幼崽在PND 21断奶。在PND 5、7和30处死各组动物。与对照组（PND 5和30时为0%，PND 7时为2%）相比，暴露于MA的动物视网膜出血百分比增加（PND 5、7和30时分别为18%、7%和11%）。在PND 30时，暴露于MA的雄性大鼠平均体重为雄性对照组平均体重的75%，而雌性大鼠的平均体重为雌性对照组的70%。这些发现支持这样一种观点，即大鼠的发育参数受到早期暴露于MA的有害影响。
