Maran R R, Aruldhas M M, Udhayakumar R C, Subramanian S, Rajendiran G, Antony F F, Arunakaran J, Govindarajulu P
Department of Endocrinology, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, India.
Int J Androl. 1998 Jun;21(3):121-8. doi: 10.1046/j.1365-2605.1998.00094.x.
The impact of hyper- and hypothyroidism on prostatic glycosidases was investigated. Hyper-thyroidism was induced by administering L-thyroxine (25 micrograms/100 g body weight/day) for 60 days and hypothyroidism was induced by total thyroidectomy. To test the direct influence of thyroid hormones, prostatic lobes were incubated with different concentrations (10, 25 and 50 ng/mL) of T3 and beta-N-acetylglucosaminidase and beta-N-acetylgalactosaminidase were assayed. Serum levels of thyroid hormones, oestradiol and testosterone increased in hyperthyroid, and decreased in hypothyroid rats. TSH decreased in hyperthyroid, and an opposite trend was seen in thyroidectomized, rats. Prostatic [anterior (coagulating glands), dorsolateral and ventral prostates] beta-glucosidase, beta-galactosidase, beta-N-acetylglucosaminidase and beta-N-acetylgalactosaminidase activities increased uniformly in hyperthyroid, and decreased in thyroidectomized, rats. In vitro studies showed a dose-dependent stimulatory effect of T3 on beta-N-acetylglucosaminidase and beta-N-acetylgalactosaminidase in all three lobes of the prostate. From the present study, it is concluded that hyperthyroidism augments and hypothyroidism inhibits prostatic glycosidases and T3 has a direct stimulatory effect on these enzymes.
研究了甲状腺功能亢进和减退对前列腺糖苷酶的影响。通过给予左旋甲状腺素（25微克/100克体重/天）60天诱导甲状腺功能亢进，通过全甲状腺切除术诱导甲状腺功能减退。为了测试甲状腺激素的直接影响，将前列腺叶与不同浓度（10、25和50纳克/毫升）的T3一起孵育，并测定β-N-乙酰氨基葡萄糖苷酶和β-N-乙酰半乳糖苷酶。甲状腺功能亢进大鼠的血清甲状腺激素、雌二醇和睾酮水平升高，甲状腺功能减退大鼠的这些水平降低。甲状腺功能亢进大鼠的促甲状腺激素降低，而甲状腺切除大鼠则呈现相反趋势。前列腺[前部（凝固腺）、背外侧前列腺和腹侧前列腺]的β-葡萄糖苷酶、β-半乳糖苷酶、β-N-乙酰氨基葡萄糖苷酶和β-N-乙酰半乳糖苷酶活性在甲状腺功能亢进大鼠中均升高，而在甲状腺切除大鼠中降低。体外研究表明，T3对前列腺所有三个叶中的β-N-乙酰氨基葡萄糖苷酶和β-N-乙酰半乳糖苷酶具有剂量依赖性刺激作用。从本研究得出结论，甲状腺功能亢进增强而甲状腺功能减退抑制前列腺糖苷酶，并且T3对这些酶具有直接刺激作用。
