Hori H, Yoshida K, Fukazawa H, Kiso Y, Sayama N, Mori K, Aizawa Y, Tani J I, Ito S
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Thyroid. 1998 Jun;8(6):525-31. doi: 10.1089/thy.1998.8.525.
Individuals with hyperthyroidism exhibit concentrations of carbonic anhydrase I (CAI) in red blood cells that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, triiodothyronine T3, at a physiological free concentration, decreases the CAI concentration in both human erythroleukemic YN-1 cells and burst-forming unit-erythroid (BFU-E)-derived cells. In the present study, the effect of T3 on CAI mRNA levels in various human erythroleukemic cell lines (YN-1, HEL and KU-812) and BFU-E-derived cells was studied. Northern analysis of RNA extracted from erythroid cells revealed a CAI mRNA of 1.5 kilobases. T3 significantly decreased the levels of CAI mRNA in YN-1 and BFU-E-derived cells in a dose-dependent manner. Incubation of T3-stimulated cells with actinomycin D prevented the decrease in CAI mRNA levels. By contrast, T3 had no effect on either the concentrations of CAI or the levels of CAI mRNA in HEL and KU-812 cells. These results suggest that YN-1 and BFU-E-derived cells may be useful models for investigating T3 actions on CAI mRNA in human cells.
甲状腺功能亢进症患者红细胞中的碳酸酐酶I（CAI）浓度反映了前几个月血清甲状腺激素的综合浓度。此外，生理游离浓度的三碘甲状腺原氨酸T3可降低人红白血病YN-1细胞和红系爆式集落形成单位（BFU-E）来源细胞中的CAI浓度。在本研究中，研究了T3对各种人红白血病细胞系（YN-1、HEL和KU-812）以及BFU-E来源细胞中CAI mRNA水平的影响。对从红系细胞中提取的RNA进行Northern分析，发现CAI mRNA为1.5千碱基。T3以剂量依赖的方式显著降低YN-1细胞和BFU-E来源细胞中CAI mRNA的水平。用放线菌素D处理T3刺激的细胞可阻止CAI mRNA水平的降低。相比之下，T3对HEL和KU-812细胞中的CAI浓度或CAI mRNA水平均无影响。这些结果表明，YN-1细胞和BFU-E来源细胞可能是研究T3对人细胞中CAI mRNA作用的有用模型。
