Harrison L C, King-Roach A P
Metabolism. 1976 Oct;25(10):1095-101. doi: 10.1016/0026-0495(76)90017-2.
The effect of varying concentrations of insulin on 1-14C-glucose conversion to 14CO2 was measured in subcutaneous adipose tissue samples obtained from 16 obese human subjects (10 nondiabetic, 6 diabetic). An index of insulin sensitivity in vitro, Kins, was calculated as the concentration of insulin stimulating one-half maximal 14CO2 production. An index of insulin sensitivity in vivo, Kitt, was calculated as the rate constant for decrease in blood glucose after rapid intravenous administration of 0.05 U/kg insulin. There was, over-all, a significant correlation between Kins and Kitt, indicating that insulin sensitivity of 1-14C-glucose oxidation by adipose tissue in vitro reflects the general state of sensitivity of glucose metabolism to insulin in vivo in obese human subjects. The mean values for both Kins and Kitt in the nondiabetic subjects were significantly different from those in the diabetic subjects, indicating greater sensitivity to insulin in the former group. The nondiabetic group was also distinguished by a significantly greater plasma insulin:blood glucose ratio in the oral glucose tolerance test. These results support the view that tissue insulin sensitivity as well as pancreatic beta cell response play an important role in determining glucose tolerance in obesity.
在从16名肥胖人类受试者（10名非糖尿病患者，6名糖尿病患者）获取的皮下脂肪组织样本中，测定了不同浓度胰岛素对1-14C-葡萄糖转化为14CO2的影响。体外胰岛素敏感性指数Kins的计算方法为：刺激14CO2产生达到最大值一半时的胰岛素浓度。体内胰岛素敏感性指数Kitt的计算方法为：快速静脉注射0.05 U/kg胰岛素后血糖降低的速率常数。总体而言，Kins与Kitt之间存在显著相关性，这表明体外脂肪组织中1-14C-葡萄糖氧化的胰岛素敏感性反映了肥胖人类受试者体内葡萄糖代谢对胰岛素的总体敏感状态。非糖尿病受试者的Kins和Kitt平均值与糖尿病受试者的显著不同，表明前一组对胰岛素更敏感。非糖尿病组在口服葡萄糖耐量试验中的血浆胰岛素：血糖比值也显著更高。这些结果支持了这样一种观点，即组织胰岛素敏感性以及胰腺β细胞反应在决定肥胖症患者的葡萄糖耐量方面起着重要作用。
