Margaux J, Hayem G, Palazzo E, Chazerain P, De Bandt M, Haim T, Kahn M F, Meyer O
Rheumatology Department, Bichat Teaching Hospital, Paris, France.
Rev Rhum Engl Ed. 1998 Jun;65(6):378-86.
To determine whether the anti-68 kDaU1snRNP antibody is associated with mixed connective tissue disease and not with SLE; to evaluate correlations between anti-U1snRNP titers and disease activity; and to look for associations between anti-U1snRNP specificities and specific clinical features.
40 patients with a positive double diffusion test for anti-68 kDa U1snRNP were studied, including 21 with mixed connective tissue disease, 14 with systemic lupus erythematosus and five with other connective tissue diseases. IgGs to 68 kDa U1snRNP were assayed using an ELISA. Clinical features, disease activity and antibody test findings were evaluated longitudinally in nine patients.
Both proportions of patients with anti-68 kDa U1snRNP and titers of IgG to 68 kDa U1snRNP were similar in the mixed connective tissue disease and systemic lupus erythematosus groups. Patients with mixed connective tissue disease were significantly more likely to have anti-A U1snRNP or anti-C U1snRNP than those with systemic lupus erythematosus (P < 0.03 and P < 0.04, respectively). No significant correlations were found between any of the features of mixed connective tissue disease (e.g., Raynaud's phenomenon, myositis, or sausage digits) and a specific anti-U1snRNP antibody. During follow-up (mean, seven years; range, 1-25 years), changes occurred in the anti-U1snRNP profile and in the anti-68 kDa U1snRNP titer. These changes were not correlated with disease activity.
IgGs to 68 kDa U1snRNP are not associated with a specific pattern of anti-RNP-positive connective tissue disease. No useful information can be gained by monitoring anti-68 kDa U1snRNP IgG titers over time. A Western blot profile including anti-A U1snRNP or anti-C U1snRNP indicates a high likelihood of U1snRNP-associated mixed connective tissue syndrome (MCTD).
确定抗68kDa U1snRNP抗体是否与混合性结缔组织病相关而非与系统性红斑狼疮相关；评估抗U1snRNP滴度与疾病活动度之间的相关性；以及寻找抗U1snRNP特异性与特定临床特征之间的关联。
对40例抗68kDa U1snRNP双扩散试验呈阳性的患者进行研究，其中包括21例混合性结缔组织病患者、14例系统性红斑狼疮患者和5例其他结缔组织病患者。使用酶联免疫吸附测定法检测针对68kDa U1snRNP的IgG。对9例患者的临床特征、疾病活动度和抗体检测结果进行纵向评估。
混合性结缔组织病组和系统性红斑狼疮组中抗68kDa U1snRNP的患者比例以及针对68kDa U1snRNP的IgG滴度相似。混合性结缔组织病患者比系统性红斑狼疮患者更有可能出现抗A U1snRNP或抗C U1snRNP（分别为P < 0.03和P < 0.04）。未发现混合性结缔组织病的任何特征（如雷诺现象、肌炎或腊肠样指）与特定的抗U1snRNP抗体之间存在显著相关性。在随访期间（平均7年；范围1 - 25年），抗U1snRNP谱和抗68kDa U1snRNP滴度发生了变化。这些变化与疾病活动度无关。
针对68kDa U1snRNP的IgG与抗RNP阳性结缔组织病的特定模式无关。随着时间推移监测抗68kDa U1snRNP IgG滴度无法获得有用信息。包含抗A U1snRNP或抗C U1snRNP的蛋白质印迹谱表明存在U1snRNP相关混合性结缔组织综合征（MCTD）的可能性很高。
