Ridgway W, Fassò M, Fathman C G
Department of Medicine, Stanford University School of Medicine, CA 94305, USA.
J Immunol. 1998 Jul 15;161(2):714-20.
The low precursor frequency of Ag-specific T cells has raised significant barriers to studying the T cell response in vivo. We demonstrate that T cells up-regulate the cell surface expression of CD4 following Ag recognition, which identifies Ag-specific T cells in vitro and in vivo and allows their characterization. The CD4high cell subpopulation contains the Ag-specific population as indicated by Ag-induced proliferation and limiting dilution analyses. The use of the CD4high marker will allow analysis of the dynamics of the T cell immune response in vivo, the study of the suboptimal T cell response to Ag, and the identification of T cells which are reactive to known and unknown autoantigens.
抗原特异性T细胞的前体频率较低，这给体内T细胞反应的研究带来了重大障碍。我们证明，T细胞在识别抗原后会上调CD4的细胞表面表达，这在体外和体内均可识别抗原特异性T细胞并对其进行表征。如抗原诱导的增殖和有限稀释分析所示，CD4高细胞亚群包含抗原特异性群体。使用CD4高标记将有助于分析体内T细胞免疫反应的动态、对抗原的次优T细胞反应的研究以及识别对已知和未知自身抗原具有反应性的T细胞。
