T7 RNA聚合酶对RNA模板的转录。
Transcription of RNA templates by T7 RNA polymerase.
作者信息
Arnaud-Barbe N, Cheynet-Sauvion V, Oriol G, Mandrand B, Mallet F
机构信息
Unité Mixte 103 CNRS-bioMérieux, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon, Cédex 07, France.
出版信息
Nucleic Acids Res. 1998 Aug 1;26(15):3550-4. doi: 10.1093/nar/26.15.3550.
Abstract
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was able to use templates containing RNA-RNA duplexes under standard transcription conditions. Implications of remnants of such RNA-dependent activities for T7 DNA-dependent polymerase are discussed.
摘要
尽管T7 RNA聚合酶高度专业化，但它似乎具有广泛的依赖于DNA和RNA的特性。为了研究这种灵活性，我们测定了T7 RNA聚合酶在双链DNA启动子起始转录后转录嵌合DNA-RNA和RNA模板的能力。我们发现，T7 RNA聚合酶能够在RNA模板上起始转录，具有持续性，并且能够在标准转录条件下使用含有RNA-RNA双链体的模板。本文讨论了这种依赖RNA的活性残余对T7依赖DNA的聚合酶的影响。
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