Chernishova I N, Kondratieva T K, Sidorova E V
Research Institute for Viral Preparations, Moscow, Russia.
Immunol Lett. 1998 May;62(1):15-8. doi: 10.1016/s0165-2478(98)00032-7.
A new approach to obtain antiserum specific for Lyb5.2 allotype, was developed. Mice of DBA/2 inbred strain, bearing Lyb5.1 allotype, were made unresponsive to cellular antigens of Lyb5-CBA/N mice by specific tolerance induction. These tolerant recipients were further immunized with suspensions of splenocytes from CBA mice which contain Lyb5.2 + B lymphocytes. Thus obtained Lyb5.2-specific antiserum was cytotoxic for 25-30% of CBA or BALB/c (both Lyb5.2 + allotype) but not for CBA/N spleen cells. When splenocytes of BALB/c mice preimmunized with polyvinylpyrrolidone (T-independent type 2 antigen) were treated with this antiserum, 50% decrease in numbers of antibody-forming and antigen-induced non-specific immunoglobulin-forming cells was documented. We conclude that, during humoral immune response to T-independent type 2 antigen, not only antibody-forming cells but also non-specific immunoglobulin-forming cells are recruited from the Lyb5 + B cell pool.
开发了一种获得针对Lyb5.2同种异型特异性抗血清的新方法。携带Lyb5.1同种异型的DBA/2近交系小鼠，通过特异性耐受诱导使其对Lyb5-CBA/N小鼠的细胞抗原无反应。这些耐受受体进一步用含有Lyb5.2 + B淋巴细胞的CBA小鼠脾细胞悬液免疫。如此获得的Lyb5.2特异性抗血清对25-30%的CBA或BALB/c（均为Lyb5.2 + 同种异型）具有细胞毒性，但对CBA/N脾细胞无细胞毒性。当用该抗血清处理预先用聚乙烯吡咯烷酮（2型非依赖T细胞抗原）免疫的BALB/c小鼠的脾细胞时，抗体形成细胞和抗原诱导的非特异性免疫球蛋白形成细胞数量减少了50%。我们得出结论，在对2型非依赖T细胞抗原的体液免疫反应过程中，不仅抗体形成细胞，而且非特异性免疫球蛋白形成细胞也从Lyb5 + B细胞库中募集。
