鉴定禽传染性支气管炎病毒S1亚基中与血清型和中和特异性表位相关的氨基酸
Identification of amino acids involved in a serotype and neutralization specific epitope within the s1 subunit of avian infectious bronchitis virus.
作者信息
Moore K M, Jackwood M W, Hilt D A
机构信息
Department of Avian Medicine, University of Georgia, Athens, USA.
出版信息
Arch Virol. 1997;142(11):2249-56. doi: 10.1007/s007050050239.
Localization of neutralizing, serotype specific epitopes of infectious bronchitis virus has been difficult because these epitopes are conformationally dependent. We identified amino acids involved in a serotype specific, conformationally dependent epitope by analysis of the S1 gene of 13 monoclonal antibody-neutralization-resistant mutants. Substitutions in the predicted amino acid sequence of these mutants were located at residues 304 and/or 386. Most of the substitutions at residue 304 were from threonine to isoleucine, whereas the substitutions at residue 386 were from arginine to proline, histidine, cysteine, or tryptophan. Based on this data, it appears that AA residues at 304 and 386 on the S1 glycoprotein are involved in a virus neutralizing serotype specific epitope.
传染性支气管炎病毒中和性、血清型特异性表位的定位一直很困难，因为这些表位依赖于构象。我们通过分析13个单克隆抗体中和抗性突变体的S1基因，确定了参与血清型特异性、构象依赖性表位的氨基酸。这些突变体预测氨基酸序列中的取代位于第304和/或386位残基。第304位残基的大多数取代是从苏氨酸变为异亮氨酸，而第386位残基的取代是从精氨酸变为脯氨酸、组氨酸、半胱氨酸或色氨酸。基于这些数据，似乎S1糖蛋白上第304和386位的氨基酸残基参与了病毒中和血清型特异性表位。
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