Fowler C L, Zimmer C C, Ruktanouchai D, Zimmer S G
Department of Surgery, University of Kentucky Medical Center, Lexington 40536, USA.
Anticancer Res. 1998 May-Jun;18(3A):1393-8.
Human neuroblastoma cell lines are notoriously difficult to establish in culture and use in murine hosts.
Two new human neuroblastoma cell lines, NK and ND, were established and studied for growth patterns in nude mice, growth in soft agar, cell cycle analysis, apoptosis (Hoechst- merocyanine 540 test), metalloproteinase expression (zymograms), and morphological differentiation by dibutyryl cyclic AMP (dCAMP).
Both cell lines formed tumors in 6/9 nude mice within 5-31 days after subcutaneous inoculation, and metastases after intravenous tail vein injection. Both grew in soft agar. DCAMP induced morphologic differentiation in both, and inhibited cell culture growth without apoptosis. Zymograms of supernatants from cultures revealed 72-kDa metalloproteinase and higher molecular bands that did not change with dCAMP treatment. Cultures derived from murine metastatic foci exhibited 72, 82 and 85-kDa proteins, with strong 92-kDa bands after dCAMP treatment.
New human neuroblastoma cell lines were established that are easily used in nude mice, and express metalloproteinases.
人神经母细胞瘤细胞系在培养中极难建立，且难以用于鼠宿主。
建立了两个新的人神经母细胞瘤细胞系NK和ND，并研究了它们在裸鼠中的生长模式、软琼脂中的生长情况、细胞周期分析、凋亡（Hoechst-花青素540试验）、金属蛋白酶表达（酶谱分析）以及二丁酰环磷腺苷（dCAMP）诱导的形态分化。
皮下接种后5 - 31天内，两种细胞系在6/9只裸鼠中均形成肿瘤，经尾静脉注射后发生转移。二者均能在软琼脂中生长。dCAMP在二者中均诱导形态分化，并抑制细胞培养生长但不引起凋亡。培养上清液的酶谱显示有72 kDa金属蛋白酶及更高分子量条带，dCAMP处理后未发生变化。源自鼠转移灶的培养物显示有72、82和85 kDa蛋白，dCAMP处理后出现强92 kDa条带。
建立了易于用于裸鼠且表达金属蛋白酶的新人神经母细胞瘤细胞系。
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