TBJ murine neuroblastoma resists dibutyryl cyclic AMP-induced differentiation and effects on metalloproteinase secretion.
作者信息
Fowler C, Zimmer C, Zimmer S
机构信息
Department of Surgery, LP Markey Cancer Center, University of Kentucky Medical Center, Lexington 40536, USA.
出版信息
J Pediatr Surg. 1998 Apr;33(4):568-72. doi: 10.1016/s0022-3468(98)90318-9.
BACKGROUND
Nonmetastatic C1300 murine neuroblastoma and TBJ, a metastatic variant, exhibit different mechanisms of metastasis and invasion.
METHODS
In this study, they were examined for response to 1 mmol/L dibutyryl cyclic AMP in vitro.
RESULTS
(1) dCAMP induced morphological differentiation in 20% to 40% of C1300 cells, whereas TBJ resisted differentiation. (2) Untreated TBJ expressed 92-kDa metalloproteinase, whereas C1300 expressed none. These results were not affected by dCAMP. (3) Growth curves of untreated C1300 and TBJ were exponential. Dibutyryl CAMP induced a plateau in C1300 growth that was confirmed by increased S-phase cells by cell cycle analysis.
CONCLUSIONS
TBJ growth was unaffected by dCAMP. Apoptosis, as assayed by Hoechst-merocyanine staining test, was not involved in the growth plateau.
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