Yoshida Y, Kamitani N, Sasaki H, Kotsuji F
Department of Obstetrics and Gynecology, Fukui Medical University, Japan.
Anticancer Res. 1998 May-Jun;18(3A):1569-77.
A new cell line (YKK) was established from a primary tumor of neuroendocrine carcinoma of the endometrium. Cultured YKK cells are polygonal in shape. The modal chromosome number is 46 XX. The doubling time of the cells, after the 19th passage, is 38 hours and this cell line has been propagated continuously by serial passage over 50 passages for the past 2 years. YKK displays characteristics resembling the original tumor cell from the donor patient: it contains the neuron-specific enolase and chromogranin antibody, produces neuron-specific enolase, and is sensitive to cisplatin, carboplatin, and etoposide. When YKK cells are transplanted subcutaneously together with Matrigel into nude mice they form a very large tumor invading the subcutaneous muscular tissue. This experimental model is useful for studying the mechanism(s) regulating the invasion of this tumor into muscular tissue, and for the establishment of new anti-cancer treatments for neuroendocrine carcinoma.
一种新的细胞系（YKK）是从子宫内膜神经内分泌癌的原发性肿瘤中建立的。培养的YKK细胞呈多边形。众数染色体数为46 XX。第19代后细胞的倍增时间为38小时，在过去两年中，该细胞系已通过连续传代在50代以上持续传代培养。YKK表现出与供体患者原始肿瘤细胞相似的特征：它含有神经元特异性烯醇化酶和嗜铬粒蛋白抗体，产生神经元特异性烯醇化酶，并且对顺铂、卡铂和依托泊苷敏感。当YKK细胞与基质胶一起皮下移植到裸鼠体内时，它们会形成一个非常大的肿瘤，侵入皮下肌肉组织。该实验模型可用于研究调节该肿瘤侵入肌肉组织的机制，以及建立神经内分泌癌的新抗癌治疗方法。
