Nasu K, Matsui N, Narahara H, Tanaka Y, Takai N, Miyakawa I, Higuchi Y
Department of Obstetrics and Gynecology, Oita Medical University, Japan.
Arch Pathol Lab Med. 1998 Sep;122(9):836-41.
Uterine endometrial stromal sarcoma is a rare neoplasm with a morphology that closely resembles that of the proliferative endometrial stroma. To understand its pathologic characteristics, we established a novel cell line, MaMi, from a primary culture of an endometrial stromal sarcoma obtained from a 65-year-old Japanese woman.
We observed the morphology of MaMi cells and performed immunohistochemical analysis on the primary tumor and transplants in nude mice. Prolactin, insulin-like growth factor-binding protein-1, interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1, and fibronectin production in the culture medium of MaMi cells were also examined.
MaMi cells were shown to exhibit a fibroblast-like morphology in vitro, and they adopted a more elongated appearance in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). On injection into nude mice, the cells gave rise to subcutaneous tumors. Immunohistologically, both the primary tumor and MaMi cell-induced tumors stained positively with antibodies to neuron-specific enolase or vimentin. MaMi cells constitutively produced IL-6, IL-8, and monocyte chemoattractant protein-1 in vitro. Interleukin-1beta, (100 pmol/L), tumor necrosis factor-alpha (1 nmol/L), and lipopolysaccharide (1 microg/mL) each increased the release of IL-6, IL-8, and monocyte chemoattractant protein-1 by MaMi cells. TPA (10 nmol/L) also stimulated the production of IL-6 and IL-8 by these cells, but inhibited that of monocyte chemoattractant protein-1.
We demonstrated that MaMi cells closely resemble proliferative endometrial stromal cells not only morphologically, but also functionally. This cell line may prove valuable in understanding the role of cytokines produced by tumor cells in the pathogenesis of endometrial stromal sarcoma and may also be useful as an in vitro model of functioning endometrial stromal cells.
子宫子宫内膜间质肉瘤是一种罕见的肿瘤，其形态与增殖期子宫内膜间质极为相似。为了解其病理特征，我们从一名65岁日本女性的子宫内膜间质肉瘤原代培养物中建立了一种新的细胞系，即MaMi细胞系。
我们观察了MaMi细胞的形态，并对裸鼠体内的原发性肿瘤和移植瘤进行了免疫组织化学分析。我们还检测了MaMi细胞培养基中催乳素、胰岛素样生长因子结合蛋白-1、白细胞介素（IL）-6、IL-8、单核细胞趋化蛋白-1、细胞间黏附分子-1、E-选择素、血管细胞黏附分子-1和纤连蛋白的产生情况。
MaMi细胞在体外表现出成纤维细胞样形态，在12-O-十四酰佛波醇-13-乙酸酯（TPA）作用下，其形态变得更加细长。将这些细胞注射到裸鼠体内后，会形成皮下肿瘤。免疫组织化学结果显示，原发性肿瘤和MaMi细胞诱导的肿瘤对神经元特异性烯醇化酶或波形蛋白抗体均呈阳性染色。MaMi细胞在体外可组成性产生IL-6、IL-8和单核细胞趋化蛋白-1。白细胞介素-1β（100 pmol/L）、肿瘤坏死因子-α（1 nmol/L）和脂多糖（1 μg/mL）均可增加MaMi细胞释放IL-6、IL-8和单核细胞趋化蛋白-1。TPA（10 nmol/L）也可刺激这些细胞产生IL-6和IL-8，但抑制单核细胞趋化蛋白-1的产生。
我们证明MaMi细胞不仅在形态上，而且在功能上与增殖期子宫内膜间质细胞极为相似。该细胞系可能有助于理解肿瘤细胞产生的细胞因子在子宫内膜间质肉瘤发病机制中的作用，也可作为功能性子宫内膜间质细胞的体外模型。
