Ito K, Sasano H, Yoshida Y, Sato S, Yajima A
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
Anticancer Res. 1998 May-Jun;18(3A):1661-4.
We studied the immunolocalization of cyclins D1 and E and their corresponding partner cyclin dependent kinases (cdk), cdk4 and cdk2 in 39 cases of human endometrioid endometrial carcinoma and examined the correlations between the labeling indexes of the cyclins, cdks and clinicopathologic parameters and the clinical outcome of the patients. Cyclin D1 immunoreactivity was observed exclusively in the nuclei of tumor cells in 22/39 (56%) of the cases examined. Immunoreactivity for cyclin E, cdk2, and cdk4 was detected in carcinoma cells of 37/39 (95%), 39/39 and 36/39 cases, respectively. There were no significant correlations between the labeling indices of any of the parameters examined. Cyclins D1 and E labeling indices were not significantly correlated with any of the clinicopathologic parameters examined. However, there was a significant correlation between cdk2 labeling index and the histological grade of carcinoma (p < 0.0001), and a significant correlation (p = 0.015) was also detected between the cdk4 labeling index and pathologic stages. There was no significant difference in clinical outcome of the patients according to the cyclin and ckd4 immunostaining patterns. These results indicate that cdk2 and cdk4 overexpression may be involved in the development and/or progression of human endometrioid endometrial carcinoma.
我们研究了细胞周期蛋白D1和E及其相应的伴侣细胞周期蛋白依赖性激酶（cdk），即cdk4和cdk2在39例人子宫内膜样腺癌中的免疫定位，并检测了细胞周期蛋白、cdk的标记指数与临床病理参数及患者临床结局之间的相关性。在所检查的39例病例中，有22例（56%）仅在肿瘤细胞核中观察到细胞周期蛋白D1免疫反应性。细胞周期蛋白E、cdk2和cdk4的免疫反应性分别在37/39例（95%）、39/39例和36/39例癌细胞中检测到。所检查的任何参数的标记指数之间均无显著相关性。细胞周期蛋白D1和E的标记指数与所检查的任何临床病理参数均无显著相关性。然而，cdk2标记指数与癌组织学分级之间存在显著相关性（p<0.0001），cdk4标记指数与病理分期之间也存在显著相关性（p = 0.015）。根据细胞周期蛋白和cdk4免疫染色模式，患者的临床结局无显著差异。这些结果表明，cdk2和cdk4的过表达可能参与了人子宫内膜样腺癌的发生和/或进展。
