López-Molina N, Laconcha I, Rementería A, Audicana A, Perales I, Garaizar J
Microbiology Division, Health Department, Basque Government, Bilbao, Spain.
J Appl Microbiol. 1998 May;84(5):877-82. doi: 10.1046/j.1365-2672.1998.00425.x.
Fifty-nine isolates of Salmonella spp. were typed by PCR fingerprinting using three single primers: ERIC2, M13 and OPS-19. First, their discrimination power in a group of nine different serotypes were studied and considerable differences in the band patterns were obtained. Further, a panel of 51 isolates of Salmonella enteritidis with eight different phage types were analysed with the three primers. The discriminating power increased by combining the patterns of the three primers, and in this case it was possible to distinguish between some phage types of Salm. enteritidis, but not all of them were differentiated.
使用三种单引物ERIC2、M13和OPS-19，通过PCR指纹图谱技术对59株沙门氏菌进行分型。首先，研究了它们在一组9种不同血清型中的鉴别能力，获得了条带模式的显著差异。此外，用这三种引物分析了一组51株具有8种不同噬菌体类型的肠炎沙门氏菌分离株。通过组合三种引物的模式，鉴别能力有所提高，在这种情况下，有可能区分一些肠炎沙门氏菌的噬菌体类型，但并非所有噬菌体类型都能区分开来。
