Rothman A, Mann D M, Behling C A, Konopka R G, Chiles P G, Pedersen C A, Moser K M
Department of Pediatrics, University of California San Diego, USA.
Chest. 1998 Jul;114(1):241-50. doi: 10.1378/chest.114.1.241.
The aims of this study were: to evaluate the performance of a novel arterial biopsy catheter in obtaining pulmonary endovascular samples in hypertensive dogs; to compare the results of pulmonary endoarterial biopsy in hypertensive vs normotensive dogs; and to assess the histologic changes in the hypertensive model.
Thirty-four dogs (27 with normal pulmonary arterial pressures and seven with pulmonary hypertension) were catheterized through an external jugular vein to obtain endovascular biopsy samples from distal pulmonary arteries 2 to 3 mm in luminal diameter. To induce pulmonary hypertension, seven dogs were given repeated infusions of 0.6- to 0.9-mm ceramic microspheres into the superior vena cava. Endoarterial samples were obtained at pulmonary systolic arterial pressures ranging from 10 to 110 mm Hg.
Sixty-two biopsy catheterization procedures were performed in the 34 dogs. After 12 initial procedures of technique refinement, endoarterial samples were obtained in each of the last 50 procedures (21 in normotensive dogs and 29 in hypertensive dogs). The average number of endovascular biopsy samples retrieved was 7.1 (range, 2 to 12) from a mean of 8.6 (range, 2 to 15) biopsy attempts per catheterization (success rate=83%). The average biopsy piece measured 1.13 mm in length, 0.33 mm in depth, and up to 1.0 mm in width. The biopsy success rates and endoarterial sample sizes were similar in normotensive and hypertensive dogs. Smooth muscle cells and endothelial cells were grown from the biopsy samples. There were no significant procedural complications, except for one self-limited hemorrhage. Histologically, samples obtained from dogs with pulmonary hypertension showed characteristic changes when compared with biopsies from normotensive dogs.
This new endoarterial biopsy catheter was safe and effective when used to obtain pulmonary endoarterial samples in dogs with normal and experimentally elevated pulmonary arterial pressures. The quality and quantity of the biopsy samples allowed identification of pathologic changes.
本研究的目的是：评估一种新型动脉活检导管在高血压犬获取肺血管内样本的性能；比较高血压犬与正常血压犬肺动脉内活检的结果；以及评估高血压模型中的组织学变化。
34只犬（27只肺动脉压力正常，7只患有肺动脉高压）通过颈外静脉插管，以从管腔直径为2至3毫米的远端肺动脉获取血管内活检样本。为诱导肺动脉高压，7只犬经上腔静脉反复输注0.6至0.9毫米的陶瓷微球。在肺动脉收缩压为10至110毫米汞柱范围内获取动脉内样本。
对34只犬进行了62次活检插管操作。在最初12次技术改进操作后，在最后50次操作中的每次操作均获取了动脉内样本（正常血压犬21次，高血压犬29次）。每次插管平均活检尝试8.6次（范围为2至15次），平均获取血管内活检样本数为7.1个（范围为2至12个）（成功率 = 83%）。平均活检组织块长度为1.13毫米，深度为0.33毫米，宽度达1.0毫米。正常血压犬和高血压犬的活检成功率及动脉内样本大小相似。从活检样本中培养出了平滑肌细胞和内皮细胞。除一例自限性出血外，无明显操作并发症。组织学上，与正常血压犬的活检相比，肺动脉高压犬获取的样本显示出特征性变化。
这种新型动脉内活检导管用于在肺动脉压力正常和实验性升高的犬中获取肺动脉内样本时安全有效。活检样本的质量和数量能够识别病理变化。
