Waldvogel H J, Fritschy J M, Mohler H, Faull R L
Department of Anatomy with Radiology, Faculty of Medicine and Health Science, University of Auckland, New Zealand.
J Comp Neurol. 1998 Aug 3;397(3):297-325. doi: 10.1002/(sici)1096-9861(19980803)397:3<297::aid-cne1>3.0.co;2-y.
The distribution of gamma-aminobutyric acid(A) (GABA(A)) receptors was investigated in the basal ganglia in the baboon brain by using receptor autoradiography and the immunohistochemical localisation of the alpha1 and beta2,3 subunits of the GABA(A) receptor by light and electron microscopy. In the caudate-putamen, the alpha1 subunit was distributed in high densities in the matrix compartment, and the beta2,3 subunits were more homogeneously distributed; the globus pallidus showed lower levels of the alpha1 and beta2,3 subunits. Four types of alpha1 subunit immunoreactive neurons were identified in the baboon striatum: the most numerous (75%) were type 1 medium-sized aspiny neurons; type 2 (2%) were large aspiny neurons with an indented nuclear membrane located in the ventral striatum; type 3 neurons were the least numerous (1%) and were comprised of large neurons in the ventromedial regions of the striatum; and type 4 (22%) neurons were medium to large aspiny neurons located in striosomes. At the ultrastructural level, alpha1 and beta2,3 subunit immunoreactivity was localised in the neuropil of the striatum in both symmetrical and asymmetrical synaptic contacts. In the globus pallidus, alpha1 and beta2,3 subunits were localised on large neurons and were found in three types of synaptic terminals: type 1 terminals were small and established symmetrical synapses; type 2 terminals were large; and type 3 terminals formed small synaptic terminals with subjunctional dense bodies. These results show that the subunit composition of GABA(A) receptors varies between the striosome and the matrix compartments in the striatum and that there is receptor subunit homogeneity in the globus pallidus.
通过受体放射自显影技术以及利用光镜和电镜对γ-氨基丁酸A（GABA(A)）受体α1和β2,3亚基进行免疫组织化学定位，研究了狒狒脑基底神经节中GABA(A)受体的分布情况。在尾状核-壳核中，α1亚基在基质区高密度分布，而β2,3亚基分布更为均匀；苍白球中α1和β2,3亚基水平较低。在狒狒纹状体中鉴定出四种类型的α1亚基免疫反应性神经元：数量最多的（75%）是1型中等大小无棘神经元；2型（2%）是位于腹侧纹状体的核膜有凹陷的大无棘神经元；3型神经元数量最少（1%），由纹状体腹内侧区域的大神经元组成；4型（22%）神经元是位于纹状体内的中等至大的无棘神经元。在超微结构水平上，α1和β2,3亚基免疫反应性定位于纹状体的神经毡中，存在于对称和不对称突触接触中。在苍白球中，α1和β2,3亚基定位于大神经元上，并存在于三种类型的突触终末：1型终末小，形成对称突触；2型终末大；3型终末形成带有突触下致密体的小突触终末。这些结果表明，GABA(A)受体的亚基组成在纹状体的纹状体内和基质区之间存在差异，并且苍白球中存在受体亚基同质性。
