Wood S J, Shewry K, Young C, Slater C R
Department of Neurobiology, School of Neurosciences, The Medical School, University of Newcastle upon Tyne, UK.
Neuroreport. 1998 Jun 22;9(9):1991-5. doi: 10.1097/00001756-199806220-00014.
Voltage-gated sodium channels (VGSCs) are concentrated in the postsynaptic membrane at the adult rat neuromuscular junction (NMJ). We have used immunolabelling to determine the pattern of initial VGSC accumulation during development. At birth, but not 3 days before, VGSC labelling is detectable at the NMJ and in the perijunctional (periJ) membrane but not elsewhere. A much higher density cluster of VGSCs forms at the NMJ itself 1-2 weeks later. If the nerve is cut 2 days after birth, VGSC labelling persists in the periJ region for at least 4 weeks but the clustering of VGSCs at the NMJ fails to develop. Thus an early, stable accumulation of VGSCs develops near the NMJ at least a week before high density postsynaptic VGSC clusters form.
电压门控钠通道（VGSCs）集中在成年大鼠神经肌肉接头（NMJ）的突触后膜中。我们利用免疫标记来确定发育过程中VGSCs初始积累的模式。出生时，而非出生前3天，在神经肌肉接头处和接头周围（periJ）膜中可检测到VGSCs标记，但在其他地方未检测到。1 - 2周后，在神经肌肉接头自身形成了密度高得多的VGSCs簇。如果在出生后2天切断神经，VGSCs标记在接头周围区域持续至少4周，但神经肌肉接头处的VGSCs簇无法形成。因此，在高密度突触后VGSCs簇形成前至少一周，在神经肌肉接头附近就出现了早期、稳定的VGSCs积累。
