Salli U, Bartol F F, Wiley A A, Tarleton B J, Braden T D
Department of Anatomy, Physiology & Pharmacology, Auburn University, Alabama 36849-5520, USA.
Biol Reprod. 1998 Jul;59(1):77-83. doi: 10.1095/biolreprod59.1.77.
Communication between cells of the corpus luteum (CL) is thought to be necessary for normal luteal function. Keratinocyte growth factor (KGF) is produced by mesenchymally derived cells in numerous tissues and acts on epithelial cells. In bovine follicles, theca cells produce KGF, which can stimulate granulosa cell proliferation. Whether KGF is produced by ovarian cells after luteinization is unknown. Our objective was to determine whether KGF mRNA and protein were present in bovine luteal tissue, and if so, to determine what type(s) of luteal cells contains KGF. CL (n = 3-4/day) were obtained from specific days throughout diestrus. Presence of KGF mRNA in CL was determined using a porcine KGF anti-sense cRNA probe. Northern analyses of luteal tissue poly(A)+ RNA revealed a single transcript (approximately 2.0 kilobases), the quantity of which did not change throughout diestrus. Western analysis revealed an immunoreactive band (28 kDa) in luteal tissues and theca cell homogenates that was absent from granulosa cell homogenates. Immunocytochemistry showed KGF predominantly in theca and small luteal cells. Results indicate that bovine CL produce and contain KGF, which is primarily localized in small luteal cells. Therefore, KGF may participate in paracrine communication within the bovine CL.
黄体（CL）细胞间的通讯被认为是黄体正常功能所必需的。角质形成细胞生长因子（KGF）由许多组织中来源于间充质的细胞产生，并作用于上皮细胞。在牛卵泡中，卵泡膜细胞产生KGF，其可刺激颗粒细胞增殖。黄体化后卵巢细胞是否产生KGF尚不清楚。我们的目的是确定牛黄体组织中是否存在KGF mRNA和蛋白，如果存在，则确定哪种类型的黄体细胞含有KGF。在整个间情期的特定日子获取CL（每天n = 3 - 4个）。使用猪KGF反义cRNA探针确定CL中KGF mRNA的存在情况。对黄体组织多聚腺苷酸加尾（poly(A)+）RNA进行Northern分析，发现一条单一转录本（约2.0千碱基），其数量在整个间情期没有变化。Western分析显示黄体组织和卵泡膜细胞匀浆中有一条免疫反应带（28 kDa），而颗粒细胞匀浆中没有。免疫细胞化学显示KGF主要存在于卵泡膜细胞和小黄体细胞中。结果表明，牛CL产生并含有KGF，其主要定位于小黄体细胞中。因此，KGF可能参与牛CL内的旁分泌通讯。
