Willats W G, Marcus S E, Knox J P
Centre for Plant Biochemistry and Biotechnology, University of Leeds, UK.
Carbohydr Res. 1998 Mar;308(1-2):149-52. doi: 10.1016/s0008-6215(98)00070-6.
A neoglycoprotein (a heptasaccharide of (1-->5)-alpha-L-linked-arabinosyl residues linked to bovine serum albumin) has been used to generate a rat monoclonal antibody specific to a linear chain of (1-->5)-alpha-L-arabinan which is a structural feature of the side chains of pectins. The antibody, designated LM6, detected 100 ng of debranched sugar beet arabinan in an immunodot binding assay and 1 microgram of commercial citrus pectin in a similar assay. Hapten inhibition studies indicated that the antibody recognized 5-6 Ara residues and 50% inhibition of antibody binding in a competitive inhibition ELISA was achieved with ca. 2ng (21 nM) of (1-->5)-alpha-L-Arabinohexaose. The antibody will be useful for the localization of arabinans in plant tissue and will have uses in the analyses of pectin structure. We report here on the localization of the arabinan epitope in lemon fruits using tissue printing.
一种新糖蛋白（一种由与牛血清白蛋白相连的(1→5)-α-L-连接阿拉伯糖基残基的七糖）已被用于产生一种大鼠单克隆抗体，该抗体对(1→5)-α-L-阿拉伯聚糖的线性链具有特异性，而(1→5)-α-L-阿拉伯聚糖是果胶侧链的一种结构特征。该抗体命名为LM6，在免疫斑点结合试验中能检测到100 ng去分支的甜菜阿拉伯聚糖，在类似试验中能检测到1 μg商业柑橘果胶。半抗原抑制研究表明，该抗体识别5 - 6个阿拉伯糖残基，在竞争性抑制酶联免疫吸附测定中，约2 ng（21 nM）的(1→5)-α-L-阿拉伯六糖可实现50%的抗体结合抑制。该抗体将有助于在植物组织中定位阿拉伯聚糖，并可用于果胶结构分析。我们在此报告使用组织印迹法在柠檬果实中定位阿拉伯聚糖表位的情况。
