Wang S, Holyoak G R, Liu G, Bunch T J, Evans R C, Bunch T D
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-9400, USA.
Anim Reprod Sci. 1998 May 15;51(3):205-13. doi: 10.1016/s0378-4320(98)00072-4.
Resazurin is a redox dye (7-hydroxy-3H-phenoxazin-3-one-10-oxide) used for assessing potential fertility of spermatozoa and functional status of eukaryotic cells. In this study, the fertilizing capacity of spermatozoa treated with resazurin and effects of resazurin on bovine embryo development in vitro was examined. Abattoir-derived bovine oocytes were collected and subjected to in vitro maturation (IVM), fertilization (IVF) and culture (IVC). In Experiment 1, bovine oocytes (n = 2767) were fertilized with spermatozoa exposed to resazurin (17.6 micrograms/ml) for 0, 15, 30, 60 min, respectively. There was no significant (P > 0.05) difference in cleavage rates. However, the proportion of embryos that developed into blastocysts, expanded and hatched blastocysts in those groups in which oocytes/embryos were treated with resazurin during IVC or IVM/IVF/IVC was significantly (P < 0.05) less than those exposed to resazurin during IVM only, or during IVF only. We conclude that resazurin did not have significant adverse effects on fertilizing capability of bovine spermatozoa; however, extended treatment of embryos with resazurin may be detrimental to embryonic development.
刃天青是一种氧化还原染料（7-羟基-3H-吩恶嗪-3-酮-10-氧化物），用于评估精子的潜在受精能力和真核细胞的功能状态。在本研究中，检测了经刃天青处理的精子的受精能力以及刃天青对牛胚胎体外发育的影响。采集屠宰场来源的牛卵母细胞，进行体外成熟（IVM）、受精（IVF）和培养（IVC）。在实验1中，分别用暴露于刃天青（17.6微克/毫升）0、15、30、60分钟的精子使牛卵母细胞（n = 2767）受精。卵裂率没有显著（P > 0.05）差异。然而，在IVC期间或IVM/IVF/IVC期间用刃天青处理卵母细胞/胚胎的那些组中，发育成囊胚、扩张囊胚和孵化囊胚的胚胎比例显著（P < 0.05）低于仅在IVM期间或仅在IVF期间暴露于刃天青的组。我们得出结论，刃天青对牛精子的受精能力没有显著不利影响；然而，用刃天青延长处理胚胎可能对胚胎发育有害。
