Dumontier S, Berche P
INSERM U411, Laboratoire de Microbiologie, Faculté de Médecine Necker-Enfants Malades, Paris, France.
FEMS Microbiol Lett. 1998 Jul 1;164(1):91-8. doi: 10.1111/j.1574-6968.1998.tb13072.x.
The new epidemic strain O139 of Vibrio cholerae, the etiologic agent of cholera, has probably emerged from the pandemic strain O1 E1 Tor through a genetic rearrangement involving the horizontal transfer of exogenous O-antigen- and capsule-encoding genes of unknown origin. In V. cholerae O139, these genes are associated with an insertion sequence designated IS1358O139. In this work, we studied the distribution of seven genes flanking the IS1358O139 element in 13 serovars of V. cholerae strains. All these O139 genes and an IS1358 element designated IS1358O22-1 were only found in V. cholerae O22 with a similar genetic organization. Sequence analysis of a 4.5-kb fragment containing IS1358O22-1 and the adjacent genes revealed that these genes are highly homologous to those of V. cholerae O139. These results suggest that strains of V. cholerae O22 from the environment might have been the source of the exogenous DNA resulting in the emergence of the new epidemic strain O139.
霍乱的病原体霍乱弧菌新流行菌株O139，可能是通过涉及未知来源的外源性O抗原和荚膜编码基因水平转移的基因重排，从大流行菌株O1埃尔托生物型演变而来。在霍乱弧菌O139中，这些基因与一个名为IS1358O139的插入序列相关。在这项研究中，我们研究了霍乱弧菌菌株13个血清型中IS1358O139元件侧翼的7个基因的分布情况。所有这些O139基因以及一个名为IS1358O22 - 1的IS1358元件，仅在具有相似基因组织的霍乱弧菌O22中发现。对包含IS1358O22 - 1及其相邻基因的4.5kb片段进行序列分析发现，这些基因与霍乱弧菌O139的基因高度同源。这些结果表明，环境中的霍乱弧菌O22菌株可能是导致新流行菌株O139出现的外源性DNA的来源。
