rfaD基因座：霍乱弧菌O139中的一个重排区域。

The rfaD locus: a region of rearrangement in Vibrio cholerae O139.

作者信息

Vimont S, Dumontier S, Escuyer V, Berche P

机构信息

INSERM U.411, Faculté de Médecine Necker-Enfants Malades, Paris, France.

出版信息

Gene. 1997 Jan 31;185(1):43-7. doi: 10.1016/s0378-1119(96)00625-7.

DOI:10.1016/s0378-1119(96)00625-7

PMID:9034311

Abstract

We analyzed the rfaD locus of the novel epidemic Vibrio cholerae strain O139, a putative region of rearrangement. This region includes 4 orfs in the same orientation. Two orfs, rfaD(O139) and orf2(O139) were almost identical to those described in V. cholerae O1. In contrast, the two other orfs upstream from rfaD(O139), designated orfA(O139) and orfB(O139), were absent from V. cholerae O1, but present in environmental strains of V. cholerae O22, O141 and O155. These results suggest that a chromosomal rearrangement might have occurred in the vicinity of rfaD in V. cholerae O1, resulting in the emergence of V. cholerae O139. The putative source of exogenous DNA might have been V. cholerae O22, O141 and O155.

摘要

我们分析了新型流行霍乱弧菌O139菌株的rfaD基因座，这是一个推测的重排区域。该区域包含4个同向的开放阅读框（orf）。其中两个开放阅读框，即rfaD（O139）和orf2（O139），与霍乱弧菌O1中描述的那些几乎相同。相比之下，rfaD（O139）上游的另外两个开放阅读框，即orfA（O139）和orfB（O139），在霍乱弧菌O1中不存在，但在霍乱弧菌O22、O141和O155的环境菌株中存在。这些结果表明，霍乱弧菌O1中rfaD附近可能发生了染色体重排，导致了霍乱弧菌O139的出现。推测的外源DNA来源可能是霍乱弧菌O22、O141和O155。