Microplate-array diagonal-gel electrophoresis (MADGE) and melt-MADGE: tools for molecular-genetic epidemiology.
作者信息
Day I N, Spanakis E, Palamand D, Weavind G P, O'Dell S D
机构信息
Wessex Human Genetics Institute, University of Southampton, UK.
出版信息
Trends Biotechnol. 1998 Jul;16(7):287-90. doi: 10.1016/s0167-7799(98)01217-7.
PMID:9675913
Abstract
Microplate-array diagonal-gel electrophoresis (MADGE) was invented for molecular-genetic epidemiological studies. It combines direct compatibility with microplates, convenient polyacrylamide-gel electrophoresis and economy of time and reagents at minimal capital cost, and enables one user to run up to several-thousand gel lanes per day for the direct assay of single-base variations. Melt-MADGE adds temporal-thermal-ramp apparatus to achieve similar throughput for de novo mutation scanning.
摘要
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