Shu M
Department of Pathology, Wellington School of Medicine.
N Z Dent J. 1998 Jun;94(416):62-4.
The object of this project was to develop a laboratory model system to study root and enamel caries. Model plaque biofilms containing consortia of four (Streptococcus mutans, Streptococcus sobrinus, Actinomyces naeslundii, and Lactobacillus rhamnosus) or six (S mutans, S sobrinus, S mitis, A naeslundii, L rhamnosus, and Veillonella dispar) species of caries pathogens were developed in a artificial mouth. Caries lesions similar to those found in vivo were produced on enamel and dentine blocks and in intact root tissues by these consortia more effectively than biofilms of the individual species. Fluoride supplied at 5 ppm suppressed enamel caries effectively, less so dentinal lesion formation, but much more so than F incorporated into the mineralised surface of enamel and roots. This is the first direct comparison of the effect on caries of environmental F and tooth mineral F induced by a plaque-like bacterial biofilm.
本项目的目的是开发一个实验室模型系统来研究牙根龋和牙釉质龋。在人工口腔中构建了含有四种（变形链球菌、远缘链球菌、内氏放线菌和鼠李糖乳杆菌）或六种（变形链球菌、远缘链球菌、轻链球菌、内氏放线菌、鼠李糖乳杆菌和殊异韦荣球菌）致龋病原体菌群的模型菌斑生物膜。与单一菌种的生物膜相比，这些菌群在牙釉质块、牙本质块以及完整牙根组织上更有效地产生了与体内发现的类似的龋损。以5 ppm提供的氟化物能有效抑制牙釉质龋，对牙本质龋损形成的抑制作用较小，但比对融入牙釉质和牙根矿化表面的氟的抑制作用要强得多。这是首次直接比较菌斑样细菌生物膜诱导的环境氟和牙齿矿物质氟对龋齿的影响。
