Filoche S K, Anderson S A, Sissons C H
Dental Research Group, Department of Pathology and Molecular Medicine, Wellington School of Medicine, Wellington, New Zealand.
Oral Microbiol Immunol. 2004 Oct;19(5):322-6. doi: 10.1111/j.1399-302x.2004.00164.x.
The ability of oral bacteria to integrate within a biofilm is pivotal to their survival. A dependence on the amount of biofilm growth by noncoaggregating Lactobacillus rhamnosus and Lactobacillus plantarum on coculture with Actinomyces naeslundii, Actinomyces gerencseriae, Streptococcus mutans and Veillonella parvula was investigated using an artificial-mouth culture system. Biofilm formation by the lactobacilli in mono-culture was poor. In coculture with Actinomyces species the amount of L. rhamnosus increased 7-20 times and L. plantarum 4-7 times compared to its mono-culture biofilm. S. mutans also promoted substantial biofilm growth of lactobacilli but V. parvula had no effect. We conclude that these Actinomyces species promoted growth of key Lactobacillus species in a biofilm, as did S. mutans to a smaller extent, and that the ability of individual bacteria to form mono-culture biofilms is not necessarily an indicator of their survival and pathogenic potential in a complex multispecies biofilm community.
口腔细菌整合到生物膜中的能力对其生存至关重要。使用人工口腔培养系统，研究了非聚集性鼠李糖乳杆菌和植物乳杆菌与内氏放线菌、杰氏放线菌、变形链球菌和小韦荣球菌共培养时对生物膜生长量的依赖性。乳杆菌在单培养中的生物膜形成较差。与放线菌属共培养时，鼠李糖乳杆菌的量比其单培养生物膜增加了7至20倍，植物乳杆菌增加了4至7倍。变形链球菌也促进了乳杆菌生物膜的大量生长，但小韦荣球菌没有影响。我们得出结论，这些放线菌属促进了生物膜中关键乳杆菌属的生长，变形链球菌在较小程度上也有此作用，而且单个细菌形成单培养生物膜的能力不一定是其在复杂多物种生物膜群落中生存和致病潜力的指标。
