Miller P, Kendall F, Nicolini C
Nucleic Acids Res. 1976 Aug;3(8):1875-81. doi: 10.1093/nar/3.8.1875.
Thermal denaturation of chromatin is observed by simultaneously monitoring absorption and circular dichroism at 276 nm as functions of temperature. Either observation indicates that sheared chromatins shows less thermal stability than native chromatin. The temperature-dependent ellipticities at 276 nm of these chromatins show features not seen in the absorption curves: the ellipticity of unsheared chromatin increases with temperature, while this increase is abolished or greatly reduced in the same chromatin after shearing. After its first thermal transition (prior to the helix-coli transition) the unsheared chromatin achieves the same ellipticity as sheared chromatin.
通过同时监测276nm处的吸光度和圆二色性随温度的变化,观察染色质的热变性。两种观察结果均表明,剪切后的染色质比天然染色质表现出更低的热稳定性。这些染色质在276nm处的温度依赖性椭圆率呈现出吸收曲线中未出现的特征:未剪切染色质的椭圆率随温度升高而增加,而在剪切后的相同染色质中,这种增加被消除或大大降低。在其第一次热转变(在螺旋-卷曲转变之前)后,未剪切染色质达到与剪切染色质相同的椭圆率。