Kazmierowski J A, Durbin W A, Reynolds H Y
Proc Soc Exp Biol Med. 1976 Sep;152(4):493-8. doi: 10.3181/00379727-152-39425.
Radiolabeled 125I Preparations of canine IgG, IgM, and F(ab')2 fragments of IgG were injected intramuscularly into normal dogs to quantitate the transfer of immunoglobulins from the intravascular space into the lower respiratory tract and to observe the clearance of these proteins from lung secretions. Respiratory fluids were sampled by serial broncho-alveolar lavages during a 3-4 week interval. Only a small portion (0.15%) of the passively administered IgG was recovered in respiratory specimens indicating that minimal transfer of humoral IgG occurs under normal conditions. Alteration of the IgG molecule by removal of the Fc portion almost eliminates its penetration into lung secretions. Furthermore, IgM, which is not a prominent component of normal lung secretions, is not detected in these secretions following passive intramuscular injection.
将放射性标记的犬IgG、IgM以及IgG的F(ab')2片段制剂经肌肉注射到正常犬体内，以定量免疫球蛋白从血管内空间向呼吸道下部的转移，并观察这些蛋白质从肺分泌物中的清除情况。在3 - 4周的间隔期内，通过连续支气管肺泡灌洗采集呼吸液样本。在呼吸标本中仅回收了一小部分（0.15%）被动给予的IgG，这表明在正常情况下，体液IgG的转移极少。通过去除Fc部分改变IgG分子几乎消除了其进入肺分泌物的情况。此外，IgM并非正常肺分泌物的主要成分，在被动肌肉注射后，这些分泌物中未检测到IgM。
