Song J, Yamamoto K, Shomura A, Itadani H, Zhong H S, Yano M, Sasaki T
Rice Genome Research Program, Institute of the Society for Techno-Innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
DNA Res. 1998 Apr 30;5(2):95-101. doi: 10.1093/dnares/5.2.95.
To understand the functions of rice homologues of the Arabidopsis flowering-time gene CONSTANS (CO) and salt-tolerance gene STO, we performed a similarity search of the single-run sequence data of cDNA clones accumulated by the Rice Genome Research Program, and isolated seven rice cDNA clones (S3574, C60910, S12569, R2931, R1479, R1577, and E10707) coding for proteins containing one of two zinc-finger-like motifs. Comparison of the deduced amino acid sequences between these cDNAs and the CO gene revealed significant similarities (46%-61%) in the region of zinc-finger motifs. A domain having a high content of basic amino acids at the C-terminus of the CO protein was found in the corresponding region of proteins predicted by from cDNAs S3574, C60910, and S12569. Two amino acid sequences, "CCADEAAL" and "FCV(L)EDRA," which were present inside each zinc-finger in the Arabidposis regulatory protein STO, were also found in each of the two zinc-finger regions of proteins predicted from cDNAs R2931, R1479, R1577, and E10707. Using restriction fragment length polymorphism (RFLP) linkage analysis, we determined the chromosomal location of the seven cDNA clones. The position of R2931 on the RFLP linkage map was closely linked to Hd-3, one of the putative quantitative trait loci (QTL) controlling heading date in rice.
为了解拟南芥开花时间基因CONSTANS（CO）和耐盐基因STO的水稻同源基因的功能，我们对水稻基因组研究计划积累的cDNA克隆单轮序列数据进行了相似性搜索，并分离出7个水稻cDNA克隆（S3574、C60910、S12569、R2931、R1479、R1577和E10707），它们编码的蛋白质含有两种锌指样基序之一。这些cDNA与CO基因推导的氨基酸序列比较显示，在锌指基序区域有显著相似性（46%-61%）。在由cDNA S3574、C60910和S12569预测的蛋白质相应区域中，发现CO蛋白C端有一个碱性氨基酸含量高的结构域。在拟南芥调控蛋白STO每个锌指内部存在的两个氨基酸序列“CCADEAAL”和“FCV(L)EDRA”，也在由cDNA R2931、R1479、R1577和E10707预测的蛋白质的两个锌指区域中各自发现。利用限制性片段长度多态性（RFLP）连锁分析，我们确定了这7个cDNA克隆的染色体定位。R2931在RFLP连锁图谱上的位置与Hd-3紧密连锁，Hd-3是控制水稻抽穗期的一个假定数量性状位点（QTL）。
