Yeast permeabilization as a tool for measurement of in situ enzyme activity: localization of alkaline phosphatase.
作者信息
Spasova D, Galabova D
机构信息
Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
出版信息
Z Naturforsch C J Biosci. 1998 May-Jun;53(5-6):347-51. doi: 10.1515/znc-1998-5-608.
PMID:9679325
Abstract
The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.
摘要
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