Inagaki H, Kitano S, Lin K H, Maeda S, Saito T
Department of Chemistry, National Industrial Research Institute of Nagoya, Japan.
Biochem Mol Biol Int. 1998 Jul;45(3):567-73. doi: 10.1080/15216549800202962.
Mitochondrial transcription factor A (mtTFA) plays an important role in regulating the expression of mitochondrial genes. To gain a better understanding of the relationships between mitochondrial gene expression and mtTFA, the mtTFA gene was inserted into a mammalian expression vector, both in the sense orientation and in the antisense orientation. After construction, these plasmids were transfected into COS-7 cells. In antisense-transformed cells, the expression of the cytochrome c oxidase subunit I and subunit III genes encoded by mitochondrial DNA was inhibited and there was an accompanying reduction of the level of mtTFA protein. These results provide direct evidence that the expression of mitochondrial genes is under the control of mtTFA.
线粒体转录因子A（mtTFA）在调节线粒体基因表达中起重要作用。为了更好地理解线粒体基因表达与mtTFA之间的关系，将mtTFA基因以正义方向和反义方向插入哺乳动物表达载体。构建完成后，将这些质粒转染到COS-7细胞中。在反义转化细胞中，线粒体DNA编码的细胞色素c氧化酶亚基I和亚基III基因的表达受到抑制，同时mtTFA蛋白水平也随之降低。这些结果提供了直接证据，证明线粒体基因的表达受mtTFA的控制。
