Yamada O, Takanashi M, Ujihara M, Mizoguchi H
Department of Hematology, Tokyo Women's Medical College, Japan.
Leuk Res. 1998 Aug;22(8):711-7. doi: 10.1016/s0145-2126(98)00065-4.
With the use of three different hematopoietic cell lineages, the downregulation of telomerase activity was found to be a general response to the induction of differentiation. The decrease in telomerase activity occurred as early as 24 h when HL-60 and K562 cells were cultured in the presence of 1alpha, 25 dihydroxyvitamin D3 (VD3), all-trans-retinoic acid (ATRA) and hemin, and completely disappeared after 3 days. On the other hand, MEG-01 cells showed a marked inhibition of telomerase activity after 6 days of culture with 12-0-tetradecanoylphorbal 13-acetate (TPA). The analysis of telomeric DNA in the HL-60 cells and K562 cells demonstrated no detectable loss of telomeric DNA with cellular differentiation, with a loss of telomerase activity. The repression of telomerase is a common molecular event during leukemic cell differentiation.
通过使用三种不同的造血细胞谱系，发现端粒酶活性的下调是诱导分化的普遍反应。当HL-60和K562细胞在1α,25-二羟基维生素D3（VD3）、全反式维甲酸（ATRA）和血红素存在的情况下培养时，端粒酶活性早在24小时就开始下降，并在3天后完全消失。另一方面，MEG-01细胞在用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）培养6天后，端粒酶活性受到显著抑制。对HL-60细胞和K562细胞中的端粒DNA分析表明，随着细胞分化，端粒DNA没有可检测到的丢失，但端粒酶活性丧失。端粒酶的抑制是白血病细胞分化过程中的一个常见分子事件。
