Jiang H, Sugimoto K, Sawada H, Takashita E, Tohma M, Gonda H, Mori K J
Department of Molecular and Cellular Biology, Faculty of Science, Niigata University, Niigata, Japan.
Blood. 1998 Aug 1;92(3):834-41.
A stroma-dependent cell line (HB-1) was established from myelogenous leukemic cells of CBA/N mouse. Characterization of the cells showed that HB-1 proliferated on hematopoietic supportive stromal cells (MS-10), but did not survive or proliferate on hematopoietic nonsupportive cells (MS-K). Direct contact between HB-1 and MS-10 appears to be necessary for HB-1 to proliferate on MS-10. We found that interleukin-1alpha (IL-1alpha) produced by MS-10 plays a major role in the survival and proliferation of HB-1. IL-11 did not support the proliferation of HB-1 cells by itself, but enhanced the proliferation of HB-1 cells in the presence of IL-1alpha. The expression of IL-1alpha and IL-11 was induced in MS-10 by the direct contact with HB-1 cells, and the expression of IL-1 receptor type I (IL-1RI) and interleukin-11 receptor (IL-11R) was induced in HB-1 cells by the attachment of the cells to MS-10. These findings show the existence of two-way interactions between HB-1 and MS-10.
从CBA/N小鼠的骨髓性白血病细胞中建立了一种基质依赖性细胞系（HB-1）。对这些细胞的特性分析表明，HB-1在造血支持性基质细胞（MS-10）上增殖，但在造血非支持性细胞（MS-K）上不能存活或增殖。HB-1与MS-10之间的直接接触似乎是HB-1在MS-10上增殖所必需的。我们发现，MS-10产生的白细胞介素-1α（IL-1α）在HB-1的存活和增殖中起主要作用。IL-11自身不能支持HB-1细胞的增殖，但在IL-1α存在的情况下能增强HB-1细胞的增殖。通过与HB-1细胞直接接触，MS-10中IL-1α和IL-11的表达被诱导，而通过细胞附着于MS-10，HB-1细胞中I型白细胞介素-1受体（IL-1RI）和白细胞介素-11受体（IL-11R）的表达被诱导。这些发现表明HB-1与MS-10之间存在双向相互作用。
