Landgraf W W, Ross P F
U. S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010, USA.
J AOAC Int. 1998 Jul-Aug;81(4):844-7.
Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.
莫能菌素用甲醇从饲料中提取，并通过溶剂分配固相萃取进行纯化。溶剂蒸发后，莫能菌素在硅胶上用薄层色谱法分离，并用香草醛显色可视化。在提交的验证研究或同行实验室分析空白样品时，未获得假阳性结果。20个添加5 ppm莫能菌素的样品中有3个报告未含有莫能菌素。所有添加10 ppm莫能菌素的样品报告莫能菌素呈阳性。
