Bozić F, Forcić D, Mazuran R, Marinculić A, Kozarić Z, Stojcević D
Department of Pharmacology and Toxicology, Veterinary Faculty, University of Zagreb, Croatia.
Comp Immunol Microbiol Infect Dis. 1998 Jul;21(3):201-14. doi: 10.1016/s0147-9571(98)00014-9.
To assess the gamma delta TCR T cells in the control of the timing of the mucosal response to enteric parasitic infections, we used C57BL mice, orally infected with 200 viable T. spiralis larvae. The small intestine, spleens and Peyer's patches (PP) were excised on 1, 4, 7, 14, 21 and 29 postinfection days (p.i.) for immunophenotyping and histological studies. Uninfected mice served as control. Characterization of isolated lymphocytes of C57BL control mice, confirmed that T cell immunophenotype differs in spleen, PP and i-IEL. Practically all i-IEL were CD3+ cells (83%). In addition, most of the i-IEL expressed Ly-2 (65%). Among the i-IEL, the level of gamma delta TCR+ cells was significantly higher (29%) than that found in spleen (3%) and PP (3%). The expression was high on CD3+ and Ly-2+ (26 and 21%, respectively) and low on L3T4+ i-IEL (< 1%). During T. spiralis infection alpha beta TCR+ CD3+, gamma delta TCR+ CD3+ and gamma delta TCR+ Ly-2+ i-IEL increased on day 4 and 7. However, infected mice displayed a reduction in i-IEL number from 14 to 29 p.i. day. At the same time the proportion of gamma delta TCR on spleen Ly-2+ and on PP CD3+ and Ly-2+ cells increased on 14 and 21 p.i. day. Adult worms were expelled from the gut by day 14. Thus, the kinetics of gamma delta TCR+ i-IEL, but not spleen and PP gamma delta TCR, corresponded to the kinetics of worm expulsion in C57BL mice. Most murine i-IEL of the gamma delta T cell lineage tend to be cytolytic when activated. We speculated that gamma delta T cells of i-IEL during the early stages of infection recognize and eliminate damaged epithelial cells generated by parasite antigens, simultaneously accelerating the worm expulsion.
为了评估γδT细胞受体T细胞在控制肠道对肠道寄生虫感染的黏膜反应时机中的作用，我们使用了经口感染200条活旋毛虫幼虫的C57BL小鼠。在感染后1、4、7、14、21和29天（p.i.）切除小肠、脾脏和派伊尔结（PP），用于免疫表型分析和组织学研究。未感染的小鼠作为对照。对C57BL对照小鼠分离淋巴细胞的特征分析证实，T细胞免疫表型在脾脏、PP和肠内上皮内淋巴细胞（i-IEL）中有所不同。实际上所有的i-IEL都是CD3+细胞（83%）。此外，大多数i-IEL表达Ly-2（65%）。在i-IEL中，γδT细胞受体+细胞的水平显著高于脾脏（3%）和PP（3%）中的水平（29%）。在CD3+和Ly-2+细胞上表达较高（分别为26%和21%），而在L3T4+ i-IEL上表达较低（<1%）。在旋毛虫感染期间，αβT细胞受体+ CD3+、γδT细胞受体+ CD3+和γδT细胞受体+ Ly-2+ i-IEL在第4天和第7天增加。然而，感染小鼠在感染后14至29天i-IEL数量减少。同时，脾脏Ly-2+以及PP CD3+和Ly-2+细胞上γδT细胞受体的比例在感染后14天和21天增加。成虫在第14天从肠道排出。因此，γδT细胞受体+ i-IEL的动力学，而非脾脏和PP的γδT细胞受体的动力学，与C57BL小鼠中蠕虫排出的动力学相对应。大多数γδT细胞谱系的小鼠i-IEL在激活时倾向于具有细胞溶解作用。我们推测，感染早期i-IEL中的γδT细胞识别并清除由寄生虫抗原产生的受损上皮细胞，同时加速蠕虫排出。
