Chen G T, Getsios S, MacCalman C D
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
Endocrinology. 1998 Aug;139(8):3512-9. doi: 10.1210/endo.139.8.6186.
Cadherin-11 (cad-11) is a novel member of the cadherin gene superfamily of calcium-dependent cell adhesion molecules. To date, the factors capable of regulating this cell adhesion molecule remain poorly characterized. We have recently determined that cad-11 expression in the human endometrium is tightly regulated during the menstrual cycle. The spatiotemporal expression of cad-11 in the stromal cells of the human endometrium during the menstrual cycle suggests that gonadal steroids regulate the expression of this endometrial cell adhesion molecule. In view of these observations, we have examined the ability of progestins, estrogens, and androgens, alone or in combination, to regulate cad-11 expression in isolated human endometrial stromal cells using Northern and Western blot analyses. In these studies, we have determined that progesterone, but not 17beta-estradiol or dihydrotestosterone, is capable of regulating cad-11 messenger RNA and protein expression levels in isolated endometrial stromal cells. In addition, 17beta-estradiol, but not dihydrotestosterone, was capable of potentiating the stimulatory effects of progesterone in a dose-dependent manner. Taken together, these observations suggest that both 17beta-estradiol and progesterone are required for maximal cad-11 expression in human endometrial stromal cells in vitro.
钙黏蛋白-11（cad-11）是钙依赖性细胞黏附分子钙黏蛋白基因超家族的一个新成员。迄今为止，能够调节这种细胞黏附分子的因素仍未得到充分表征。我们最近确定，在月经周期中，人子宫内膜中cad-11的表达受到严格调控。月经周期中cad-11在人子宫内膜基质细胞中的时空表达表明，性腺类固醇调节这种子宫内膜细胞黏附分子的表达。鉴于这些观察结果，我们使用Northern和Western印迹分析，研究了孕激素、雌激素和雄激素单独或联合调节分离的人子宫内膜基质细胞中cad-11表达的能力。在这些研究中，我们确定，孕酮能够调节分离的子宫内膜基质细胞中cad-11信使核糖核酸和蛋白质的表达水平，而17β-雌二醇或二氢睾酮则不能。此外,17β-雌二醇能够以剂量依赖的方式增强孕酮的刺激作用，而二氢睾酮则不能。综上所述，这些观察结果表明，在体外人子宫内膜基质细胞中，最大程度的cad-11表达需要17β-雌二醇和孕酮共同作用。
