心房利钠肽基因多态性与糖尿病肾病。糖尿病肾病研究组。

A polymorphism in the gene for the atrial natriuretic peptide and diabetic nephropathy. Diabetic Nephropathy Study Group.

作者信息

Schmidt S, Blüthner M, Giessel R, Strojek K, Bergis K H, Grzeszczak W, Ritz E

机构信息

Department of Internal Medicine, Ruprecht Karls University of Heidelberg, Germany.

出版信息

Nephrol Dial Transplant. 1998 Jul;13(7):1807-10. doi: 10.1093/ndt/13.7.1807.

DOI:10.1093/ndt/13.7.1807

PMID:9681733

Abstract

BACKGROUND

Atrial natriuretic peptide is involved in blood pressure regulation via its vasodilating and natriuretic actions. Since diabetic nephropathy and hypertension are closely related, ANP is a reasonable candidate gene for diabetic nephropathy (DN).

METHODS

We genotyped 410 patients with type I diabetes (without DN n = 307; with DN n = 103) and 658 patients with type II diabetes (without DN n = 464; with DN n = 194). In the patients the duration of diabetes was at least 10 years. Diabetic nephropathy was defined as urinary albumin excretion of > or = 30 mg/24 h. The HpaII polymorphism in intron 2 of the ANP gene was determined using PCR amplification followed by restriction digest. Alleles were separated on agarose gels stained with ethidium bromide.

RESULTS

We compared genotype distribution and allele frequencies between patients with and without nephropathy. No significant difference was observed either in type I (allele frequency without DN H1, 0.02/H2, 0.98 vs with DN H1, 0.05/H2, 0.95; P = 0.132) or in type II diabetes (allele frequency without DN H1, 0.04/H2, 0.96 vs with DN H1, 0.05/H2, 0.95; P = 0.551).

CONCLUSIONS

The polymorphism in the gene for the atrial natriuretic peptide does not seem to play a major role in the development of diabetic nephropathy in either type I or in type II diabetes.

摘要

背景

心房利钠肽通过其血管舒张和利钠作用参与血压调节。由于糖尿病肾病与高血压密切相关，心房利钠肽（ANP）是糖尿病肾病（DN）的一个合理候选基因。

方法

我们对410例1型糖尿病患者（无糖尿病肾病n = 307；有糖尿病肾病n = 103）和658例2型糖尿病患者（无糖尿病肾病n = 464；有糖尿病肾病n = 194）进行基因分型。这些患者的糖尿病病程至少为10年。糖尿病肾病定义为尿白蛋白排泄量≥30 mg/24 h。使用聚合酶链反应（PCR）扩增后进行限制性酶切来确定ANP基因第2内含子中的HpaII多态性。等位基因在溴化乙锭染色的琼脂糖凝胶上分离。

结果

我们比较了有肾病和无肾病患者之间的基因型分布和等位基因频率。在1型糖尿病（无糖尿病肾病的等位基因频率H1，0.02/H2，0.98；有糖尿病肾病的等位基因频率H1，0.05/H2，0.95；P = 0.132）或2型糖尿病（无糖尿病肾病的等位基因频率H1，0.04/H2，0.96；有糖尿病肾病的等位基因频率H1，0.05/H2，0.95；P = 0.551）中均未观察到显著差异。