Boatto G, Cerri R, Pau A, Palomba M, Pintore G, Denti M G
Istituto di Analitica Farmaceutica, Facoltà di Farmacia, Università degli Studi di Sassari, Italy.
J Pharm Biomed Anal. 1998 Aug;17(4-5):733-8. doi: 10.1016/s0731-7085(97)00245-8.
A method for determination in vivo of the benzylpenicillin residues in ovine milk at low levels was described. Two groups of Sardinian breed sheep were treated with a dose of penicillin G sodium salt by intramammary infusion and intramuscular administration respectively. The residues were detected by isocratic HPLC method of the extract obtained from a previous cleanup procedure. Linear calibration plots were obtained over a large concentration range of 1 mg ml-1 -10 ng ml-1, with correlation coefficients greater than 0.998. Recoveries between 78.6 and 87.3% were obtained. Limit of detection (LOD) and limit of determination (LOQ) were 2.6 and 8.8 ng ml-1 respectively. This method would be useful for routine monitoring of penicillin G residues in ovine dairy milk.
