一种用于测定亲水性胶体糊剂基质中两性霉素B的高效液相色谱法。
A high-performance liquid-chromatographic assay for amphotericin B in a hydrophilic colloidal paste base.
作者信息
Wilkinson J M, McDonald C, Parkin J E, Sunderland V B
机构信息
School of Pharmacy, Curtin University of Technology, Perth, Western Australia, Australia.
出版信息
J Pharm Biomed Anal. 1998 Aug;17(4-5):751-5. doi: 10.1016/s0731-7085(97)00250-1.
A stability-indicating high-performance liquid-chromatographic (HPLC) assay has been developed for amphotericin B (AmB) in a paste, containing AmB, tobramycin (or gentamicin) sulphate, colistin sulphate, liquid paraffin and Orabase. Extraction of AmB was performed by partitioning the antibiotic between N,N-dimethylformamide (DMF) and cyclohexane, which led to precipitation of the polymeric materials and extraction of the liquid paraffin into the cyclohexane and AmB into the DMF. Analysis by HPLC of the latter layer gave a linear relationship between concentration and peak area response for the AmB over the range 5.0 x 10(-4) to 7.5 x 10(-3)% (w/v) (r = 0.9995) with a relative standard deviation of +/- 1.46% (n = 8). The efficiency of extraction was 1006 +/- 2.4% (n = 5).
已开发出一种稳定性指示高效液相色谱（HPLC）分析法，用于测定含两性霉素B（AmB）、硫酸妥布霉素（或庆大霉素）、硫酸多粘菌素、液体石蜡和奥拉巴酯的糊剂中的两性霉素B。两性霉素B的提取是通过将抗生素在N，N - 二甲基甲酰胺（DMF）和环己烷之间进行分配来实现的，这导致聚合材料沉淀，液体石蜡被提取到环己烷中，而两性霉素B被提取到DMF中。对后一层进行HPLC分析，结果表明，在5.0×10⁻⁴至7.5×10⁻³%（w/v）范围内，两性霉素B的浓度与峰面积响应呈线性关系（r = 0.9995），相对标准偏差为±1.46%（n = 8）。提取效率为100±2.4%（n = 5）。
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