Zhang-Barber L, Turner A K, Dougan G, Barrow P A
Institute for Animal Health, Compton, Newbury Berkshire, UK.
Vaccine. 1998 May-Jun;16(9-10):899-903. doi: 10.1016/s0264-410x(97)00300-9.
A nuoG mutation in NADH dehydrogenase I was introduced into a virulent strain of Salmonella serotype Gallinarum, the causative agent of fowl typhoid, using gene replacement with a nuoG open reading frame inactivated by insertion of DNA encoding a kanamycin resistance determinant. The S. Gallinarum nuoG mutant, named SG9NGK, was highly attenuated in chickens. SG9NGK colonized the caeca of chickens less efficiently than the S. Gallinarum parental strain, was less invasive and showed no evidence of multiplication in the liver or spleen. Using a single oral immunization with live bacteria SG9NGK reduced mortality in 2-week-old chickens following challenge with virulent S. Gallinarum from 75% to less than 8%.
利用编码卡那霉素抗性决定簇的DNA插入使nuoG开放阅读框失活，通过基因置换将NADH脱氢酶I中的nuoG突变引入鸡伤寒病原体鸡沙门氏菌血清型Gallinarum的强毒株中。命名为SG9NGK的鸡沙门氏菌nuoG突变体在鸡中高度减毒。SG9NGK在鸡盲肠中的定殖效率低于鸡沙门氏菌亲本菌株，侵袭性较小，并且在肝脏或脾脏中没有增殖迹象。用活细菌SG9NGK进行单次口服免疫后，2周龄鸡在受到强毒鸡沙门氏菌攻击后的死亡率从75%降至不到8%。
