Saikh K U, Sesno J, Brandler P, Ulrich R G
Department of Immunology and Molecular Biology, Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702-5011, USA.
Vaccine. 1998 May-Jun;16(9-10):1029-38. doi: 10.1016/s0264-410x(97)00280-6.
Polypeptide and DNA vaccine alternatives to the conventional tetanus toxoid were compared. Mouse immunizations with plasmid DNA that encoded the tetanus toxin C fragment polypeptide induced consistently lower antibody responses than direct immunization with the C fragment polypeptide or toxoid, yet provided some degree of protection from a lethal toxin challenge. Cytotoxic T-cell responses dominated DNA immunizations, while specific T-cell proliferation resulted from all vaccines tested. Immune responses to the DNA vaccine exhibited a T-helper type-1 propensity, while polypeptides elicited T-helper type-2 responses. The lower antibody response to the plasmid vaccine was not due to insufficient quantity of C fragment in vivo but was likely the result of a mode of antigen presentation that was less efficient for supporting antibody production. Collectively, these results suggest that polypeptide or toxoid vaccines are preferable to plasmid-based vaccination for control of diseases caused by tetanus toxin.
对传统破伤风类毒素的多肽和DNA疫苗替代方案进行了比较。用编码破伤风毒素C片段多肽的质粒DNA免疫小鼠，诱导产生的抗体反应始终低于直接用C片段多肽或类毒素免疫，但能提供一定程度的保护，使其免受致死性毒素攻击。细胞毒性T细胞反应在DNA免疫中占主导，而所有测试疫苗均能引发特异性T细胞增殖。对DNA疫苗的免疫反应表现出1型辅助性T细胞倾向，而多肽引发2型辅助性T细胞反应。对质粒疫苗抗体反应较低并非由于体内C片段数量不足，而可能是抗原呈递方式效率较低，不利于支持抗体产生的结果。总体而言，这些结果表明，对于控制由破伤风毒素引起的疾病，多肽或类毒素疫苗比基于质粒的疫苗更可取。
