Gor D O, Li A C, Rosenthal P J
Department of Medicine, San Francisco General Hospital, CA, USA.
Vaccine. 1998 Jul;16(11-12):1193-202. doi: 10.1016/s0264-410x(98)80119-9.
The Plasmodium falciparum proteins serine repeat antigen (SERA) and serine repeat protein homologue (SERPH) have similarity in sequence with cysteine proteases in a well-conserved protease domain. We identified three SERA homologues from the murine malaria parasite Plasmodium vinckei and evaluated immune responses to the protease domains of these proteins. Mice that developed protective immunity to P. vinckei after serial infection and cure demonstrated humoral and cell-mediated responses against the SERA homologue protease domains. Mice immunized with Salmonella typhimurium expressing the protease domain of one of these antigens demonstrated cellular responses against the antigen and increased survival against lethal challenge with P. vinckei. Our results suggest that the protease domains of SERA and SERPH are worthy of additional study as potential components of a malaria vaccine.
恶性疟原虫蛋白丝氨酸重复抗原（SERA）和丝氨酸重复蛋白同源物（SERPH）在一个高度保守的蛋白酶结构域中与半胱氨酸蛋白酶具有序列相似性。我们从小鼠疟原虫文氏疟原虫中鉴定出三种SERA同源物，并评估了针对这些蛋白蛋白酶结构域的免疫反应。在连续感染和治愈后对文氏疟原虫产生保护性免疫的小鼠表现出针对SERA同源物蛋白酶结构域的体液和细胞介导反应。用表达其中一种抗原蛋白酶结构域的鼠伤寒沙门氏菌免疫的小鼠表现出针对该抗原的细胞反应，并提高了对文氏疟原虫致死性攻击的存活率。我们的结果表明，SERA和SERPH的蛋白酶结构域作为疟疾疫苗的潜在成分值得进一步研究。
