细胞外pH对大鼠活化小胶质细胞膜电位的调节作用

Modulation of membrane potential by extracellular pH in activated microglia in rats.

作者信息

Chung S, Lee M Y, Soh H, Jung W, Joe E

机构信息

Department of Physiology, Chung-Ang University College of Medicine, Seoul, South Korea.

出版信息

Neurosci Lett. 1998 Jun 19;249(2-3):139-42. doi: 10.1016/s0304-3940(98)00409-1.

DOI:10.1016/s0304-3940(98)00409-1

PMID:9682836

Abstract

Activation of cultured rat microglial cells by lipopolysaccharide (LPS) induced delayed rectifying outward K+ (I(K)) current. I(K) current was reported to have 'window current', playing a direct role in setting the membrane potential in activated microglia. We used whole-cell patch clamp method to measure the effect of extracellular pH on I(K) current. When pH was changed from 7.4 to 6.4, the activation curve of I(K) current shifted to the right by about 13 mV. Thus, extracellular acidification reduced the window current, resulting in membrane depolarization. These results suggest that extracellular pH regulate the membrane potential in activated microglia.

摘要

脂多糖（LPS）激活培养的大鼠小胶质细胞可诱导延迟整流外向钾离子（I(K)）电流。据报道，I(K)电流具有“窗电流”，在激活的小胶质细胞中膜电位的设定中起直接作用。我们使用全细胞膜片钳方法来测量细胞外pH对I(K)电流的影响。当pH从7.4变为6.4时，I(K)电流的激活曲线向右移动约13 mV。因此，细胞外酸化会降低窗电流，导致膜去极化。这些结果表明，细胞外pH调节激活的小胶质细胞中的膜电位。