Sancho J M
Servicio de Endocrinología, Hospital Ramon y Cajal, Madrid, Spain.
Clin Exp Hypertens. 1998 Jul-Aug;20(5-6):535-42. doi: 10.3109/10641969809053231.
We have isolated from bovine hypothalamic and pituitary tissue the sodium pump inhibitor HHIF that is structurally different from ouabain. By mass spectrometric analysis this purified factor revealed a single unique molecular ion with an accurate mass of 412.277 and a mass spectra different from ouabain. It has been previously shown that HHIF inhibits the Ca2+-ATPase of the plasma membrane of synaptosomes. HHIF increases free calcium levels in cultured rat mesangial cells as well as mesangial cell contraction and proliferation. With the same purification procedure we have isolated in parallel HHIF and Ouabain from central nervous tissue. Ouabain elutes prior to HHIF in the final purification HPLC systems. This endogenous Ouabain has, in all the systems tested, the same chromatographic behavior that synthetic cold or [3H] Ouabain.
我们已从牛下丘脑和垂体组织中分离出钠泵抑制剂HHIF，其结构与哇巴因不同。通过质谱分析，这种纯化因子显示出一个单一独特的分子离子，精确质量为412.277，质谱与哇巴因不同。先前已表明HHIF可抑制突触体质膜的Ca2 + -ATP酶。HHIF可增加培养的大鼠系膜细胞中的游离钙水平以及系膜细胞的收缩和增殖。通过相同的纯化程序，我们已从中枢神经组织中并行分离出HHIF和哇巴因。在最终纯化的HPLC系统中，哇巴因比HHIF先洗脱。在所有测试系统中，这种内源性哇巴因具有与合成冷哇巴因或[3H]哇巴因相同的色谱行为。
