海葱苷A免疫反应性：其纯化、通过特异性结合蛋白在血液中的运输及其与血压的相关性。

Proscillaridin A immunoreactivity: its purification, transport in blood by a specific binding protein and its correlation with blood pressure.

作者信息

Schneider R, Antolovic R, Kost H, Sich B, Kirch U, Tepel M, Zidek W, Schoner W

机构信息

Institut für Biochemie & Endokrinologie, Justus-Liebig-Universität, Giessen, Germany.

出版信息

Clin Exp Hypertens. 1998 Jul-Aug;20(5-6):593-9. doi: 10.3109/10641969809053237.

DOI:10.3109/10641969809053237

PMID:9682915

Abstract

A material crossreacting with antibodies against the bufadienolide proscillaridin A and inhibiting the sodium pump was found in human blood plasma. The concentration of the material with a retention time similar to ouabain in a reversed phase HPLC correlated to systolic blood pressure and pulse pressure. Affinity purification of this compound from bovine adrenals resulted in the isolation of a compound with molecular mass of 600 Da that was not identical with ouabain. Consistent with the postulate that endogenous ouabain and proscillaridin A immunoreactivities may belong to a new class of cardiotonic steroid hormones, a protein of Mr = 60 kDa has been found in bovine serum by affinity-labeling with N-hydroxysuccimidyl digoxigenin-3-O-methylcarbonyl-epsilon-aminocaproate.

摘要

在人血浆中发现了一种与抗蟾蜍二烯羟酸内酯海葱苷A抗体发生交叉反应并抑制钠泵的物质。在反相高效液相色谱中，保留时间与哇巴因相似的该物质浓度与收缩压和脉压相关。从牛肾上腺中亲和纯化该化合物，得到一种分子量为600 Da且与哇巴因不同的化合物。与内源性哇巴因和海葱苷A免疫反应性可能属于一类新型强心甾体激素的假设一致，通过用N-羟基琥珀酰亚胺地高辛-3-O-甲基羰基-ε-氨基己酸进行亲和标记，在牛血清中发现了一种分子量为60 kDa的蛋白质。