Haegele A D, Wolfe P, Thompson H J
Division of Laboratory Research, AMC Cancer Research Center, Denver, CO 80214, USA.
Carcinogenesis. 1998 Jul;19(7):1319-21. doi: 10.1093/carcin/19.7.1319.
Ionizing radiation is a carcinogen that induces oxidative DNA damage. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a relatively abundant, mutagenic lesion that is widely regarded as a reliable index of oxidative DNA damage. The purpose of this study was to examine the effects of X-radiation on levels of 8-OHdG in the context of an experimental model for breast cancer in which chronic radiation exposure has been shown to be carcinogenic in Sprague-Dawley rats. A secondary objective of this study was to determine if the use of phenol during DNA isolation affected the concentration of 8-OHdG subsequently measured. Our results indicate that a profoundly carcinogenic dose of radiation induced a small but significant increase in 8-OHdG concentration in mammary gland DNA, and that the use of a phenol-based versus a salt-based method of DNA isolation had no significant impact on the levels of 8-OHdG detected in either control or irradiated tissue.
电离辐射是一种能诱发DNA氧化损伤的致癌物。8-羟基-2'-脱氧鸟苷（8-OHdG）是一种相对常见的诱变损伤，被广泛认为是DNA氧化损伤的可靠指标。本研究的目的是在乳腺癌实验模型的背景下，研究X射线辐射对8-OHdG水平的影响，在该模型中，长期辐射暴露已被证明对Sprague-Dawley大鼠具有致癌性。本研究的第二个目的是确定在DNA分离过程中使用苯酚是否会影响随后测量的8-OHdG浓度。我们的结果表明，高致癌剂量的辐射导致乳腺DNA中8-OHdG浓度有小幅但显著的增加，并且使用基于苯酚的方法与基于盐的DNA分离方法对在对照组织或受辐照组织中检测到的8-OHdG水平没有显著影响。
