Marquez M E, Carlini A R, Slobodianik N H, Ronayne de Ferrer P A, Godoy M F
Department of Biological Sciences, Argentine Antarctic Institute, Buenos Aires, Argentina.
Comp Biochem Physiol A Mol Integr Physiol. 1998 Mar;119(3):795-9. doi: 10.1016/s1095-6433(98)01018-6.
This paper reports Immunoglobulin M (IgM) levels in serum samples from eight female-pup pairs of southern elephant seals (Mirounga leonina), at King George Island, Antarctica. IgM levels were determined on sera obtained from sequential sampling throughout the suckling period (approximately 23 days). The IgM concentration in southern elephant seal serum was measured by single radial immunodiffusion on agarose plates. Female IgM levels (123.5-613.0 mg/dL, n = 8) were significantly higher than pup levels (5.9-123.6 mg/dL, n = 8). Both groups showed an increasing trend throughout the entire suckling period, with significant differences in relation to stages of lactation. Pup IgM levels on the first day of life (mean +/- SD, 7.6 +/- 2.9 mg/dL, n = 3) suggest that endogenous synthesis takes place before birth.
本文报告了在南极乔治王岛采集的八对南象海豹（Mirounga leonina）雌性幼崽血清样本中的免疫球蛋白M（IgM）水平。IgM水平是通过在整个哺乳期（约23天）连续采样获得的血清来测定的。南象海豹血清中的IgM浓度通过琼脂糖平板上的单向放射免疫扩散法进行测量。雌性IgM水平（123.5 - 613.0毫克/分升，n = 8）显著高于幼崽水平（5.9 - 123.6毫克/分升，n = 8）。两组在整个哺乳期均呈现上升趋势，且与哺乳阶段存在显著差异。幼崽出生第一天的IgM水平（平均值±标准差，7.6±2.9毫克/分升，n = 3）表明内源性合成在出生前就已发生。
