Pho-P在枯草芽孢杆菌细胞壁阴离子聚合物生物合成相关基因转录调控中的作用
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
作者信息
Qi Y, Hulett F M
机构信息
Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, Illinois 60607, USA.
出版信息
J Bacteriol. 1998 Aug;180(15):4007-10. doi: 10.1128/JB.180.15.4007-4010.1998.
Abstract
tagA, tagD, and tuaA operons are responsible for the synthesis of cell wall anionic polymer, teichoic acid, and teichuronic acid, respectively, in Bacillus subtilis. Under phosphate starvation conditions, teichuronic acid is synthesized while teichoic acid synthesis is inhibited. Expression of these genes is controlled by PhoP-PhoR, a two-component system. It has been proposed that Pho-P plays a key role in the activation of tuaA and the repression of tagA and tagD. In this study, we demonstrated the role of Pho-P in the switch process from teichoic acid synthesis to teichuronic acid synthesis, by using an in vitro transcription system. The results indicate that PhoP approximately P is sufficient to repress the transcription of the tagA and tagD promoters and also to activate the transcription of the tuaA promoter.
摘要
在枯草芽孢杆菌中，tagA操纵子、tagD操纵子和tuaA操纵子分别负责细胞壁阴离子聚合物、磷壁酸和磷壁醛酸的合成。在磷酸盐饥饿条件下，会合成磷壁醛酸，同时抑制磷壁酸的合成。这些基因的表达受双组分系统PhoP-PhoR调控。有人提出，Pho-P在激活tuaA以及抑制tagA和tagD中起关键作用。在本研究中，我们通过使用体外转录系统证明了Pho-P在从磷壁酸合成到磷壁醛酸合成的转换过程中的作用。结果表明，PhoP-PhoR足以抑制tagA和tagD启动子的转录，也足以激活tuaA启动子的转录。
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PhoP-P and RNA polymerase sigmaA holoenzyme are sufficient for transcription of Pho regulon promoters in Bacillus subtilis: PhoP-P activator sites within the coding region stimulate transcription in vitro.PhoP-P与RNA聚合酶σA全酶足以启动枯草芽孢杆菌中Pho调控子启动子的转录：编码区内的PhoP-P激活位点在体外可刺激转录。
Mol Microbiol. 1998 Jun;28(6):1187-97. doi: 10.1046/j.1365-2958.1998.00882.x.
2
Comparison of PhoP binding to the tuaA promoter with PhoP binding to other Pho-regulon promoters establishes a Bacillus subtilis Pho core binding site.将PhoP与tuaA启动子的结合同PhoP与其他Pho调控子启动子的结合进行比较，从而确定了枯草芽孢杆菌的Pho核心结合位点。
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3
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.phoA和pstS编码区域内部的位点可结合PhoP，且是实现完整启动子活性所必需的。
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4
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.在磷酸盐饥饿期间对枯草芽孢杆菌tagAB和tagDEF表达的分析确定了PhoP-P的阻遏作用。
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